| PpAlign_Program | ppalign |
| PpAlign_Version | 1.0 |
| Algorithm | Global |
| Alphabet | protein |
| Scorematrix | blosum62 |
| Gap_Open | 11 |
| Gap_Extension | 1 |
| Transition Matrix | | P | A | B |
| S | 0.999981 | 9.71981e-06 | 9.71987e-06 |
| P | 0.999981 | 9.71981e-06 | 9.71987e-06 |
| A | 0.632121 | 0.367879 | 0 |
| B | 0.632117 | 3.88393e-06 | 0.367879 |
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| Pair Matrix | | A | R | N | D | C | Q | E | G | H | I | L | K | M | F | P | S | T | W | Y | V |
| A | 0.021528 | 0.00230299 | 0.00190247 | 0.00220286 | 0.00160208 | 0.00190247 | 0.00300391 | 0.00580755 | 0.00110143 | 0.00320417 | 0.00440573 | 0.0033043 | 0.00130169 | 0.00160208 | 0.00220286 | 0.0063082 | 0.00370482 | 0.000400521 | 0.00130169 | 0.00510664 |
| R | 0.00230299 | 0.0178232 | 0.0020026 | 0.00160208 | 0.000400521 | 0.00250325 | 0.00270351 | 0.00170221 | 0.00120156 | 0.00120156 | 0.00240312 | 0.00620807 | 0.000801041 | 0.000901172 | 0.0010013 | 0.00230299 | 0.00180234 | 0.000300391 | 0.000901172 | 0.00160208 |
| N | 0.00190247 | 0.0020026 | 0.0141184 | 0.00370482 | 0.000400521 | 0.00150195 | 0.00220286 | 0.00290377 | 0.00140182 | 0.0010013 | 0.00140182 | 0.00240312 | 0.000500651 | 0.000801041 | 0.000901172 | 0.00310404 | 0.00220286 | 0.00020026 | 0.000700911 | 0.00120156 |
| D | 0.00220286 | 0.00160208 | 0.00370482 | 0.0213277 | 0.000400521 | 0.00160208 | 0.00490638 | 0.00250325 | 0.0010013 | 0.00120156 | 0.00150195 | 0.00240312 | 0.000500651 | 0.000801041 | 0.00120156 | 0.00280364 | 0.00190247 | 0.00020026 | 0.000600781 | 0.00130169 |
| C | 0.00160208 | 0.000400521 | 0.000400521 | 0.000400521 | 0.0119155 | 0.000300391 | 0.000400521 | 0.000801041 | 0.00020026 | 0.00110143 | 0.00160208 | 0.000500651 | 0.000400521 | 0.000500651 | 0.000400521 | 0.0010013 | 0.000901172 | 0.00010013 | 0.000300391 | 0.00140182 |
| Q | 0.00190247 | 0.00250325 | 0.00150195 | 0.00160208 | 0.000300391 | 0.0073095 | 0.00350456 | 0.00140182 | 0.0010013 | 0.000901172 | 0.00160208 | 0.00310404 | 0.000700911 | 0.000500651 | 0.000801041 | 0.00190247 | 0.00140182 | 0.00020026 | 0.000700911 | 0.00120156 |
| E | 0.00300391 | 0.00270351 | 0.00220286 | 0.00490638 | 0.000400521 | 0.00350456 | 0.016121 | 0.00190247 | 0.00140182 | 0.00120156 | 0.0020026 | 0.00410534 | 0.000700911 | 0.000901172 | 0.00140182 | 0.00300391 | 0.0020026 | 0.000300391 | 0.000901172 | 0.00170221 |
| G | 0.00580755 | 0.00170221 | 0.00290377 | 0.00250325 | 0.000801041 | 0.00140182 | 0.00190247 | 0.0378492 | 0.0010013 | 0.00140182 | 0.00210273 | 0.00250325 | 0.000700911 | 0.00120156 | 0.00140182 | 0.00380495 | 0.00220286 | 0.000400521 | 0.000801041 | 0.00180234 |
| H | 0.00110143 | 0.00120156 | 0.00140182 | 0.0010013 | 0.00020026 | 0.0010013 | 0.00140182 | 0.0010013 | 0.00931211 | 0.000600781 | 0.0010013 | 0.00120156 | 0.000400521 | 0.000801041 | 0.000500651 | 0.00110143 | 0.000700911 | 0.00020026 | 0.00150195 | 0.000600781 |
| I | 0.00320417 | 0.00120156 | 0.0010013 | 0.00120156 | 0.00110143 | 0.000901172 | 0.00120156 | 0.00140182 | 0.000600781 | 0.018424 | 0.0114148 | 0.00160208 | 0.00250325 | 0.00300391 | 0.0010013 | 0.00170221 | 0.00270351 | 0.000400521 | 0.00140182 | 0.0120156 |
| L | 0.00440573 | 0.00240312 | 0.00140182 | 0.00150195 | 0.00160208 | 0.00160208 | 0.0020026 | 0.00210273 | 0.0010013 | 0.0114148 | 0.0371483 | 0.00250325 | 0.00490638 | 0.00540703 | 0.00140182 | 0.00240312 | 0.0033043 | 0.000700911 | 0.00220286 | 0.00951237 |
| K | 0.0033043 | 0.00620807 | 0.00240312 | 0.00240312 | 0.000500651 | 0.00310404 | 0.00410534 | 0.00250325 | 0.00120156 | 0.00160208 | 0.00250325 | 0.016121 | 0.000901172 | 0.000901172 | 0.00160208 | 0.00310404 | 0.00230299 | 0.000300391 | 0.0010013 | 0.00190247 |
| M | 0.00130169 | 0.000801041 | 0.000500651 | 0.000500651 | 0.000400521 | 0.000700911 | 0.000700911 | 0.000700911 | 0.000400521 | 0.00250325 | 0.00490638 | 0.000901172 | 0.00400521 | 0.00120156 | 0.000400521 | 0.000901172 | 0.0010013 | 0.00020026 | 0.000600781 | 0.00230299 |
| F | 0.00160208 | 0.000901172 | 0.000801041 | 0.000801041 | 0.000500651 | 0.000500651 | 0.000901172 | 0.00120156 | 0.000801041 | 0.00300391 | 0.00540703 | 0.000901172 | 0.00120156 | 0.0183238 | 0.000500651 | 0.00120156 | 0.00120156 | 0.000801041 | 0.00420547 | 0.00260338 |
| P | 0.00220286 | 0.0010013 | 0.000901172 | 0.00120156 | 0.000400521 | 0.000801041 | 0.00140182 | 0.00140182 | 0.000500651 | 0.0010013 | 0.00140182 | 0.00160208 | 0.000400521 | 0.000500651 | 0.0191249 | 0.00170221 | 0.00140182 | 0.00010013 | 0.000500651 | 0.00120156 |
| S | 0.0063082 | 0.00230299 | 0.00310404 | 0.00280364 | 0.0010013 | 0.00190247 | 0.00300391 | 0.00380495 | 0.00110143 | 0.00170221 | 0.00240312 | 0.00310404 | 0.000901172 | 0.00120156 | 0.00170221 | 0.0126164 | 0.00470612 | 0.000300391 | 0.0010013 | 0.00240312 |
| T | 0.00370482 | 0.00180234 | 0.00220286 | 0.00190247 | 0.000901172 | 0.00140182 | 0.0020026 | 0.00220286 | 0.000700911 | 0.00270351 | 0.0033043 | 0.00230299 | 0.0010013 | 0.00120156 | 0.00140182 | 0.00470612 | 0.0125163 | 0.000300391 | 0.000901172 | 0.00360469 |
| W | 0.000400521 | 0.000300391 | 0.00020026 | 0.00020026 | 0.00010013 | 0.00020026 | 0.000300391 | 0.000400521 | 0.00020026 | 0.000400521 | 0.000700911 | 0.000300391 | 0.00020026 | 0.000801041 | 0.00010013 | 0.000300391 | 0.000300391 | 0.00650846 | 0.000901172 | 0.000400521 |
| Y | 0.00130169 | 0.000901172 | 0.000700911 | 0.000600781 | 0.000300391 | 0.000700911 | 0.000901172 | 0.000801041 | 0.00150195 | 0.00140182 | 0.00220286 | 0.0010013 | 0.000600781 | 0.00420547 | 0.000500651 | 0.0010013 | 0.000901172 | 0.000901172 | 0.0102133 | 0.00150195 |
| V | 0.00510664 | 0.00160208 | 0.00120156 | 0.00130169 | 0.00140182 | 0.00120156 | 0.00170221 | 0.00180234 | 0.000600781 | 0.0120156 | 0.00951237 | 0.00190247 | 0.00230299 | 0.00260338 | 0.00120156 | 0.00240312 | 0.00360469 | 0.000400521 | 0.00150195 | 0.0196255 |
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| Background | | A | R | N | D | C | Q | E | G | H | I | L | K | M | F | P | S | T | W | Y | V |
| 0.0778443 | 0.0508982 | 0.0409182 | 0.0518962 | 0.0239521 | 0.0339321 | 0.0588822 | 0.0828343 | 0.0249501 | 0.0618762 | 0.0918164 | 0.0558882 | 0.0239521 | 0.0439122 | 0.0429142 | 0.0588822 | 0.0548902 | 0.0139721 | 0.0339321 | 0.0718563 |
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